Enhanced nanomagnetic gene transfection of human prenatal cardiac progenitor cells and adult cardiomyocytes
Author(s)
Subramanian, Mahendran
Lim, Jenson
Dobson, Jon
Type
Journal Article
Abstract
Magnetic nanoparticle-based gene transfection has been shown to be an effective, non-viral technique for delivery of both plasmid DNA and siRNA into cells in culture. It has several advantages over other non-viral delivery techniques, such as short transfection times and high cell viability. These advantages have been demonstrated in a number of primary cells and cell lines. Here we report that oscillating magnet array-based nanomagnetic transfection significantly improves transfection efficiency in both human prenatal cardiac progenitor cells and adult cardiomyocytes when compared to static magnetofection, cationic lipid reagents and electroporation, while maintaining high cell viability. In addition, transfection of adult cardiomyocytes was improved further by seeding the cells onto Collagen I-coated plates, with transfection efficiencies of up to 49% compared to 24% with lipid reagents and 19% with electroporation. These results demonstrate that oscillating nanomagnetic transfection far outperforms other non-viral transfection techniques in these important cells.
Date Issued
2013-07-31
Date Acceptance
2013-06-12
Citation
PLoS One, 2013, 8 (7), pp.1-8
ISSN
1932-6203
Publisher
Public Library of Science (PLoS)
Start Page
1
End Page
8
Journal / Book Title
PLoS One
Volume
8
Issue
7
Copyright Statement
© 2013 Subramanian et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000322633700046&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
MAGNETIC NANOPARTICLES
IN-VIVO
STEM-CELLS
DELIVERY
MAGNETOFECTION
THERAPY
HEART
EFFICIENCY
DNA
OPTIMIZATION
Publication Status
Published
Article Number
ARTN e69812
Date Publish Online
2013-07-31