Engineering strategies for producing medium-long chain dicarboxylic acids in oleaginous yeasts
Author(s)
Type
Journal Article
Abstract
Medium-long chain dicarboxylic acids (DCAs, C ≥ 6) are essential chemical raw materials, with wide applications in the chemical, pharmaceutical, material and food industries. However, the traditional chemical synthesis methods cause environmental pollution and are not in line with goals of sustainable development. With the development of synthetic biology, high-value-added DCAs can be biosynthesized from hydrophobic substrates (HSs) using suitable microorganisms. This review first summarizes the biosynthetic pathway of DCAs in oleaginous yeasts and then emphasizes the related engineering strategies for increasing the product yield, including promoter, enzyme, pathway, cell, fermentation, and downstream engineering. In addition, the challenges and development trends in the biosynthesis of DCAs are discussed, in light of the current progress, challenges, and trends in this field. Finally, guidelines for future research are proposed. Overall, this review systematically summarizes recent engineering strategies for DCAs production in oleaginous yeasts and offers valuable insights for future DCAs biosynthesis.
Date Issued
2025-08-01
Date Acceptance
2025-04-25
Citation
Bioresource Technology, 2025, 430
ISSN
0960-8524
Publisher
Elsevier
Journal / Book Title
Bioresource Technology
Volume
430
Copyright Statement
Copyright © 2025 Elsevier Ltd. This is the author’s accepted manuscript made available under a CC-BY licence in accordance with Imperial’s Research Publications Open Access policy (www.imperial.ac.uk/oa-policy)
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/40294756
PII: S0960-8524(25)00559-0
Subjects
Agricultural Engineering
Agriculture
ALKANE-ASSIMILATING YEAST
ALPHA,OMEGA-DICARBOXYLIC ACIDS
BETA-OXIDATION
Biotechnology & Applied Microbiology
CANDIDA-TROPICALIS
CYP52 FAMILY
DIRECTED EVOLUTION
Energy & Fuels
FATTY ALCOHOL OXIDASE
Hydrophobic substrates
Life Sciences & Biomedicine
MULTIGENE FAMILY
NONANOIC ACID
omega-Oxidation
Science & Technology
Synthetic biology
Technology
Yarrowia lipolytica
YARROWIA-LIPOLYTICA
Publication Status
Published
Coverage Spatial
England
Article Number
132593
Date Publish Online
2025-04-26