Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform
File(s)
Accepted version
Author(s)
Malpartida-Cardenas, Kenny
Miscourides, Nicholas
Rodriguez-Manzano, Jesus
Yu, Ling-Shan
Moser, Nicolas
Type
Journal Article
Abstract
Early and accurate diagnosis of malaria and drug-resistance is essential to effective disease management. Available rapid malaria diagnostic tests present limitations in analytical sensitivity, drug-resistance testing and/or quantification. Conversely, diagnostic methods based on nucleic acid amplification stepped forwards owing to their high sensitivity, specificity and robustness. Nevertheless, these methods commonly rely on optical measurements and complex instrumentation which limit their applicability in resource-poor, point-of-care settings. This paper reports the specific, quantitative and fully-electronic detection of Plasmodium falciparum, the predominant malaria-causing parasite worldwide, using a Lab-on-Chip platform developed in-house. Furthermore, we demonstrate on-chip detection of C580Y, the most prevalent single-nucleotide polymorphism associated to artemisinin-resistant malaria. Real-time non-optical DNA sensing is facilitated using Ion-Sensitive Field-Effect Transistors, fabricated in unmodified complementary metal-oxide-semiconductor (CMOS) technology, coupled with loop-mediated isothermal amplification. This work holds significant potential for the development of a fully portable and quantitative malaria diagnostic that can be used as a rapid point-of-care test.
Date Issued
2019-12-01
Date Acceptance
2019-09-04
Citation
Biosensors and Bioelectronics, 2019, 145
ISSN
0956-5663
Publisher
Elsevier BV
Journal / Book Title
Biosensors and Bioelectronics
Volume
145
Copyright Statement
© 2019 Elsevier B.V. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence http://creativecommons.org/licenses/by-nc-nd/4.0/
Sponsor
Wellcome Trust
Wellcome Trust
Engineering & Physical Science Research Council (E
Engineering & Physical Science Research Council (EPSRC)
Identifier
https://www.sciencedirect.com/science/article/pii/S0956566319307572?via%3Dihub
Grant Number
100993/Z/13/Z
100993/Z/13/Z
EP/P510798/1
EP/R512655/1
Subjects
CMOS
ISFET
LAMP
Lab-on-Chip
Malaria
P. falciparum
Point-of-Care
SNP
0301 Analytical Chemistry
0903 Biomedical Engineering
1007 Nanotechnology
Bioinformatics
Publication Status
Published
Article Number
ARTN 111678
Date Publish Online
2019-09-07