mSphere of influence: modifying an old method to study RNA-protein interactions
File(s)
Author(s)
Tiengwe, Calvin
Type
Journal Article
Abstract
Calvin Tiengwe works on posttranscriptional gene regulation and iron homeostasis in the parasitic protozoan Trypanosoma brucei. In this mSphere of Influence article, he reflects on how the paper “Comprehensive identification of RNA-protein interactions in any organism using orthogonal organic phase separation (OOPS)” by Queiroz et al. (Nat Biotechnol 37:169–178, 2019, https://doi.org/10.1038/s41587-018-0001-2) influenced his research by providing a tool to capture RNA-protein complexes on a global scale using acid guanidinium thiocyanate-phenol-chloroform (AGPC), an old method hitherto applied for RNA, DNA, or protein purification.
Date Issued
2019-09-01
Date Acceptance
2019-09-01
Citation
mSphere, 2019, 4 (5), pp.1-2
ISSN
2379-5042
Publisher
American Society for Microbiology
Start Page
1
End Page
2
Journal / Book Title
mSphere
Volume
4
Issue
5
Copyright Statement
© 2019 Tiengwe. This is an open-access article distributed under the terms of the creative Commons Attribution 4.0 International license.
License URL
Sponsor
Wellcome Trust
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000490605800031&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Grant Number
208780/Z/17/Z
Subjects
Science & Technology
Life Sciences & Biomedicine
Microbiology
AGPC
OOPS
RNA binding proteins
Publication Status
Published
Article Number
ARTN e00600-19
Date Publish Online
2019-09-04