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Enhancing the production of chlorophyll f in the cyanobacterium Synechocystis sp. PCC 6803
File(s)
Author(s)
Qi, Man
Taunt, Henry
Bečková, Martina
Xia, Zhi
Trinugroho, JP
Type
Journal Article
Abstract
One potential approach to improve the productivity of cyanobacteria and microalgae is to enhance photosynthetic efficiency by introducing far-red absorbing pigment molecules (such as chlorophylls f and d) into the photosynthetic apparatus to expand the range of photosynthetically active radiation. We have shown previously that expressing the ChlF subunit of Chroococcidiopsis thermalis PCC 7203 in the model cyanobacterium Synechocystis sp. PCC 6803 (Syn6803) is sufficient to drive the production of chlorophyll f (Chl f), but only to low levels (0.24% Chl f/Chl a). By using the strong Pcpc560 promoter and an N-terminal truncated derivative of ChlF, we have been able to increase the yield of Chl f in white light by over 30-fold to about 8.2% Chl f/Chl a, close to the level displayed by far-red photoacclimated C. thermalis 7203. Additionally, we demonstrate that ChlF from Fisherella thermalis PCC 7521, like ChlF from C. thermalis 7203, assembles into a variant of the monomeric photosystem II (PSII) core complex termed the super-rogue PSII complex when expressed in Syn6803. This contrasts with the originally reported formation of a ChlF homodimeric complex in Synechococcus sp. PCC 7002. Overall, our work is an important starting point for mechanistic and structural studies of super-rogue PSII and for incorporating Chl f into the photosynthetic apparatus of Syn6803.
Date Issued
2025-03-01
Date Acceptance
2025-02-10
Citation
Physiologia Plantarum, 2025, 177 (2)
URI
URL
DOI
ISSN
0031-9317
Publisher
Wiley
Journal / Book Title
Physiologia Plantarum
Volume
177
Issue
2
Copyright Statement
© 2025 The Author(s). Physiologia Plantarum published by John Wiley & Sons Ltd on behalf of Scandinavian Plant Physiology Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
License URL
Identifier
10.1111/ppl.70169
Publication Status
Published
Article Number
e70169
Date Publish Online
2025-03-26