Slice-illuminated optical projection tomography
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
To improve the imaging performance of optical projection tomography (OPT) in live samples, we have explored a parallelized implementation of semi-confocal line illumination and detection to discriminate against scattered photons. Slice-illuminated OPT (sl-OPT) improves reconstruction quality in scattering samples by reducing interpixel crosstalk at the cost of increased acquisition time. For in vivo imaging, this can be ameliorated through the use of compressed sensing on angularly undersampled OPT data sets. Here, we demonstrate sl-OPT applied to 3D imaging of bead phantoms and live adult zebrafish.
Date Issued
2018-11-15
Date Acceptance
2018-10-01
Citation
Optics Letters, 2018, 43 (22), pp.5555-5558
ISSN
0146-9592
Publisher
Optical Society of America
Start Page
5555
End Page
5558
Journal / Book Title
Optics Letters
Volume
43
Issue
22
Copyright Statement
© 2018 Optical Society of America. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.
Sponsor
Medical Research Council (MRC)
Grant Number
MR/K011561/1
Subjects
Science & Technology
Physical Sciences
Optics
SCATTERING MEDIA
ADULT ZEBRAFISH
MICROSCOPY
TOOL
0205 Optical Physics
0206 Quantum Physics
0906 Electrical And Electronic Engineering
Publication Status
Published