Nanoscale imaging of primary cilia with scanning ion conductance microscopy
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
Primary cilia are hair-like sensory organelles whose dimensions and location vary with cell type and culture condition. Herein, we employed scanning ion conductance microscopy (SICM) to visualize the topography of primary cilia from different cell types. By combining SICM with fluorescence imaging, we successfully distinguished between surface cilia that project outward from the cell surface and subsurface cilia that are trapped below it. The nanoscale structure of the ciliary pocket, which cannot be easily identified using a confocal fluorescence microscope, was observed in SICM images. Furthermore, we developed a topographic reconstruction method using current-distance profiles to evaluate the relationship between set point and topographic image and found that a low set point is important for detecting the true topography of a primary cilium using hopping mode SICM.
Date Issued
2018-02-20
Date Acceptance
2018-01-18
Citation
Analytical Chemistry, 2018, 90 (4), pp.2891-2895
ISSN
0003-2700
Publisher
American Chemical Society
Start Page
2891
End Page
2895
Journal / Book Title
Analytical Chemistry
Volume
90
Issue
4
Copyright Statement
© 2018 American Chemical Society
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000426143100068&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Physical Sciences
Chemistry, Analytical
Chemistry
ATOMIC-FORCE MICROSCOPY
CILIOGENESIS
CELLS
RESORPTION
Publication Status
Published
Date Publish Online
2018-01-18