easySTORM: a robust, lower-cost approach to localisation and TIRF microscopy
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Accepted version
Published version
Author(s)
Type
Journal Article
Abstract
TIRF and STORM microscopy are super-resolving fluorescence imaging modalities for which current implementations on standard microscopes can present significant complexity and cost. We present a straightforward and low-cost approach to implement STORM and TIRF taking advantage of multimode optical fibres and multimode diode lasers to provide the required excitation light. Combined with open source software and relatively simple protocols to prepare samples for STORM, including the use of Vectashield for non-TIRF imaging, this approach enables TIRF and STORM imaging of cells labelled with appropriate dyes or expressing suitable fluorescent proteins to become widely accessible at low cost.
Date Issued
2016-03-11
Date Acceptance
2016-01-31
Citation
Journal of Biophotonics, 2016, 9 (9), pp.948-957
ISSN
1864-0648
Publisher
Wiley-VCH Verlag
Start Page
948
End Page
957
Journal / Book Title
Journal of Biophotonics
Volume
9
Issue
9
Copyright Statement
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction
in any medium, provided the original work is properly cited.
in any medium, provided the original work is properly cited.
License URL
Sponsor
Wellcome Trust
Medical Research Council (MRC)
Medical Research Council
Grant Number
093465/Z/10/Z
MR/K015834/1
MR/K015834/1
Subjects
Optoelectronics & Photonics
Publication Status
Published