Lipid profiling of complex biological mixtures by liquid chromatography/mass spectrometry using a novel scanning quadrupole data-independent acquisition strategy
File(s)Lee final accepted draft.pdf (2.45 MB)
Accepted version
Author(s)
Type
Journal Article
Abstract
Rationale
A novel data-independent acquisition method is detailed that incorporates a scanning quadrupole in front of an orthogonal acceleration time-of-flight (TOF) mass analyser. This approach is described and the attributes are compared and contrasted to other DIA approaches.
Methods
Specific application of the method to both targeted and untargeted lipidomic identification strategies is discussed, with data from both shotgun and LC separated lipidomics experiments presented.
Results
The benefits of the fast quadrupole scanning technique are highlighted, and include improvements in speed and specificity for complex mixtures providing high quality qualitative and quantitative data.
Conclusions
In particular the high specificity afforded by the scanning quadrupole improves qualitative information for lipid identification.
A novel data-independent acquisition method is detailed that incorporates a scanning quadrupole in front of an orthogonal acceleration time-of-flight (TOF) mass analyser. This approach is described and the attributes are compared and contrasted to other DIA approaches.
Methods
Specific application of the method to both targeted and untargeted lipidomic identification strategies is discussed, with data from both shotgun and LC separated lipidomics experiments presented.
Results
The benefits of the fast quadrupole scanning technique are highlighted, and include improvements in speed and specificity for complex mixtures providing high quality qualitative and quantitative data.
Conclusions
In particular the high specificity afforded by the scanning quadrupole improves qualitative information for lipid identification.
Date Issued
2017-09-04
Date Acceptance
2017-07-10
Citation
Rapid Communications in Mass Spectrometry, 2017, 31 (19), pp.1599-1606
ISSN
0951-4198
Publisher
Wiley
Start Page
1599
End Page
1606
Journal / Book Title
Rapid Communications in Mass Spectrometry
Volume
31
Issue
19
Copyright Statement
This is the peer reviewed version of the following article: Gethings LA, Richardson K, Wildgoose J, et al. Lipid profiling of complex biological mixtures by liquid chromatography/mass spectrometry using a novel scanning quadrupole data-independent acquisition strategy. Rapid Commun Mass Spectrom. 2017;31:1599-1606. , which has been published in final form at https://dx.doi.org/10.1002/rcm.7941. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
Sponsor
Wellcome Trust
Genesis Research Trust
Grant Number
102758/Z/13/Z
01070
Subjects
03 Chemical Sciences
04 Earth Sciences
06 Biological Sciences
Analytical Chemistry
Publication Status
Published
Date Publish Online
2017-09-04