A method for assessing the spatiotemporal resolution of structured illumination microscopy (SIM)
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Published version
Author(s)
Boualam, Abderrahim
Rowlands, Christopher
Type
Journal Article
Abstract
A method is proposed for assessing the temporal resolution of structured illumination microscopy (SIM), by tracking the amplitude of different spatial frequency components over time, and comparing them to a temporally-oscillating ground-truth. This method is used to gain insight into the performance limits of SIM, along with alternative reconstruction techniques (termed ‘rolling SIM’) that claim to improve temporal resolution. Results show that the temporal resolution of SIM varies considerably between low and high spatial frequencies, and that, despite being used in several high profile papers and commercial microscope software, rolling SIM provides no increase in temporal resolution over conventional SIM.
Date Issued
2021-02-01
Date Acceptance
2020-12-21
Citation
Biomedical Optics Express, 2021, 12 (2), pp.790-801
ISSN
2156-7085
Publisher
Optical Society of America
Start Page
790
End Page
801
Journal / Book Title
Biomedical Optics Express
Volume
12
Issue
2
Copyright Statement
© 2021 The Author(s). Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Furtherdistribution of this work must maintain attribution to the author(s) and the published article’s title, journalcitation, and DOI.
License URL
Sponsor
Wellcome Trust
Engineering & Physical Science Research Council (EPSRC)
Grant Number
212490/Z/18/Z
EP/S016538/1
Subjects
physics.optics
physics.optics
eess.IV
0205 Optical Physics
0912 Materials Engineering
Publication Status
Published
Date Publish Online
2021-01-12