Hepatitis C virus infection of cholangiocarcinoma cell lines
File(s)
Author(s)
Type
Journal Article
Abstract
Hepatitis C virus (HCV) infects the liver and hepatocytes are the major cell type supporting viral replication. Hepatocytes and cholangiocytes derive from a common hepatic progenitor cell that proliferates during inflammatory conditions, raising the possibility that cholangiocytes may support HCV replication and contribute to the hepatic reservoir. We screened cholangiocytes along with a panel of cholangiocarcinoma-derived cell lines for their ability to support HCV entry and replication. While primary cholangiocytes were refractory to infection and lacked expression of several entry factors, two cholangiocarcinoma lines, CC-LP-1 and Sk-ChA-1, supported efficient HCV entry; furthermore, Sk-ChA-1 cells supported full virus replication. In vivo cholangiocarcinomas expressed all of the essential HCV entry factors; however, cholangiocytes adjacent to the tumour and in normal tissue showed a similar pattern of receptor expression to ex vivo isolated cholangiocytes, lacking SR-BI expression, explaining their inability to support infection. This study provides the first report that HCV can infect cholangiocarcinoma cells and suggests that these heterogeneous tumours may provide a reservoir for HCV replication in vivo.
Date Issued
2015-06-01
Date Acceptance
2015-02-07
Citation
Journal of General Virology, 2015, 96 (6), pp.1380-1388
ISSN
1465-2099
Publisher
Microbiology Society
Start Page
1380
End Page
1388
Journal / Book Title
Journal of General Virology
Volume
96
Issue
6
Copyright Statement
This is an open access article published by the Microbiology Society under the Creative Commons Attribution License
License URL
Sponsor
Dr Hadwen Trust for Humane Research
Subjects
Cell Line, Tumor
Epithelial Cells
Hepacivirus
Humans
Viral Tropism
Virus Internalization
Virus Replication
Virology
06 Biological Sciences
07 Agricultural And Veterinary Sciences
11 Medical And Health Sciences
Publication Status
Published