Sub-millisecond control of neuronal firing by organic light-emitting diodes
File(s)
Author(s)
Matarese, Bruno FE
Feyen, Paul LC
de Mello, John C
Benfenati, Fabio
Type
Journal Article
Abstract
Optogenetics combines optics and genetics to enable minimally invasive cell-type-specific stimulation in living tissue. For the purposes of bio-implantation, there is a need to develop soft, flexible, transparent and highly biocompatible light sources. Organic semiconducting materials have key advantages over their inorganic counterparts, including low Young's moduli, high strain resistances, and wide color tunability. However, until now it has been unclear whether organic light emitting diodes (OLEDs) are capable of providing sufficient optical power for successful neuronal stimulation, while still remaining within a biologically acceptable temperature range. Here we investigate the use of blue polyfluorene- and orange poly(p-phenylenevinylene)-based OLEDs as stimuli for blue-light-activated Sustained Step Function Opsin (SFFO) and red-light-activated ChrimsonR opsin, respectively. We show that, when biased using high frequency (multi-kHz) drive schemes, the OLEDs permit safe and controlled photostimulation of opsin-expressing neurons and were able to control neuronal firing with high temporal-resolution at operating temperatures lower than previously demonstrated.
Date Issued
2019-10-22
Date Acceptance
2019-10-03
Citation
Frontiers in Bioengineering and Biotechnology, 2019, 7
ISSN
2296-4185
Publisher
Frontiers Media
Journal / Book Title
Frontiers in Bioengineering and Biotechnology
Volume
7
Copyright Statement
© 2019 Matarèse, Feyen, de Mello and Benfenati. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
License URL
Identifier
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000497402900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Multidisciplinary Sciences
Science & Technology - Other Topics
organic light-emitting diodes
optogenetics
neurons
pulsed-operation
optical stimulation
bioelectronics
photoexcitation
electrophysiology
OPTOGENETIC VISION RESTORATION
ADENYLYL-CYCLASE
INHIBITION
STIMULATION
SENSITIVITY
ACTIVATION
INTERFACE
BEHAVIOR
DENSITY
DEVICES
Publication Status
Published
Article Number
ARTN 278