An experimental study was conducted into the degree of composite action that can arise between cold-formed steel joists and wood-based flooring panels. A series of material, push-out and 4-point bending tests were carried out, and alternative means of shear connection, featuring fasteners and adhesives, were investigated. It was found that the spacing of the fasteners and the application of structural adhesive at the beam-board interface had a significant influence on the attained degree of shear connection and, hence, the moment capacity and flexural stiffness of the system. The highest degree of shear connection (up to approximately 60%) was obtained using the structural adhesive, bringing corresponding increases in capacity and stiffness of approximately 100 and 40%, respectively, over the bare steel. Smaller, but still very significant, increases in capacity and stiffness were achieved through the use of screws alone. On the basis of the results of the push-out tests, a load-slip relationship for screw fasteners in wood-based floorboards was proposed; this was designed for use in future analytical and numerical models. The findings of this research demonstrate, for the first time, the benefits that can be derived through the practical exploitation of composite action in cold-formed steel flooring systems in terms of enhanced structural performance and efficiency of material use.