The “beauty in the beast”—the multiple uses of Priestia megaterium in biotechnology
File(s)
Published version
Author(s)
Biedendieck, Rebekka
Knuuti, Tobias
Moore, Simon J
Jahn, Dieter
Type
Journal Article
Abstract
<jats:secOver 30 years, the Gram-positive bacterium Priestia megaterium (previously known as Bacillus megaterium) was systematically developed for biotechnological applications ranging from the production of small molecules like vitamin B12, over polymers like polyhydroxybutyrate (PHB) up to the in vivo and in vitro synthesis of multiple proteins and finally whole-cell applications. Here we describe the use of the natural vitamin B12 (cobalamin) producer P. megaterium for the elucidation of the biosynthetic pathway and the subsequent systematic knowledge-based development for production purposes. The formation of PHB, a natural product of P. megaterium and potential petro-plastic substitute, is covered and discussed. Further important biotechnological characteristics of P. megaterium for recombinant protein production including high protein secretion capacity and simple cultivation on value-added carbon sources are outlined. This includes the advanced system with almost 30 commercially available expression vectors for the intracellular and extracellular production of recombinant proteins at the g/L scale. We also revealed a novel P. megaterium transcription-translation system as a complementary and versatile biotechnological tool kit. As an impressive biotechnology application, the formation of various cytochrome P450 is also critically highlighted. Finally, whole cellular applications in plant protection are completing the overall picture of P. megaterium as a versatile giant cell factory.
Key points
• The use of Priestia megaterium for the biosynthesis of small molecules and recombinant proteins through to whole-cell applications is reviewed.
• P. megaterium can act as a promising alternative host in biotechnological production processes.
Key points
• The use of Priestia megaterium for the biosynthesis of small molecules and recombinant proteins through to whole-cell applications is reviewed.
• P. megaterium can act as a promising alternative host in biotechnological production processes.
Date Issued
2021-08-01
Date Acceptance
2021-06-21
Citation
Applied Microbiology and Biotechnology, 2021, 105 (14-15), pp.5719-5737
ISSN
0175-7598
Publisher
Springer Science and Business Media LLC
Start Page
5719
End Page
5737
Journal / Book Title
Applied Microbiology and Biotechnology
Volume
105
Issue
14-15
Copyright Statement
© The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/34263356
PII: 10.1007/s00253-021-11424-6
Subjects
Bacillus megaterium
Cell-free transcription-translation
Cytochrome P450
Plant growth-promoting bacterium
Polyhydroxybutyrate (PHB)
Priestia megaterium
Recombinant protein production
Vitamin B12
Bacillus megaterium
Beauty
Biotechnology
Recombinant Proteins
Vitamin B 12
Publication Status
Published
Coverage Spatial
Germany
Date Publish Online
2021-07-15