DIRECT, a low-cost system for high-speed, low-noise imaging of fluorescent bio-samples
File(s)
Published version
Author(s)
Whiteley, Isabell
Song, Chenchen
Howe, Glenn A
Knöpfel, Thomas
Rowlands, Christopher J
Type
Journal Article
Abstract
A targeted imaging system has been developed for applications requiring recording from stationary samples at high spatiotemporal resolutions. It works by illuminating regions of interest in rapid sequence, and recording the signal from the whole field of view onto a single photodetector. It can be implemented at low cost on an existing microscope without compromising existing functionality. The system is characterized in terms of speed, spatial resolution, and tissue penetration depth, before being used to record individual action potentials from ASAP-3 expressing neurons in an ex vivo mouse brain slice preparation.
Date Issued
2023-06-01
Date Acceptance
2023-04-11
Citation
Biomedical Optics Express, 2023, 14 (6), pp.1-11
ISSN
2156-7085
Publisher
Optica Publishing Group
Start Page
1
End Page
11
Journal / Book Title
Biomedical Optics Express
Volume
14
Issue
6
Copyright Statement
© 2023 The Author(s). Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.
License URL
Identifier
http://dx.doi.org/10.1364/boe.486507
Publication Status
Published
Date Publish Online
2023-05-08