Intercellular movement of the putative transcription factor SHR in root patterning
File(s)
Accepted version
Author(s)
Nakajima, K
Sena, G
Nawy, T
Benfey, PN
Type
Journal Article
Abstract
Positional information is pivotal for establishing developmental patterning in plants1,2,3, but little is known about the underlying signalling mechanisms. The Arabidopsis root radial pattern is generated through stereotyped division of initial cells and the subsequent acquisition of cell fate4. short-root (shr) mutants do not undergo the longitudinal cell division of the cortex/endodermis initial daughter cell, resulting in a single cell layer with only cortex attributes5,6. Thus, SHR is necessary for both cell division and endodermis specification5,6. SHR messenger RNA is found exclusively in the stele cells internal to the endodermis and cortex, indicating that it has a non-cell-autonomous mode of action6. Here we show that the SHR protein, a putative transcription factor, moves from the stele to a single layer of adjacent cells, where it enters the nucleus. Ectopic expression of SHR driven by the promoter of the downstream gene SCARECROW (SCR) results in autocatalytic reinforcement of SHR signalling, producing altered cell fates and multiplication of cell layers. These results support a model in which SHR protein acts both as a signal from the stele and as an activator of endodermal cell fate and SCR-mediated cell division.
Date Issued
2001-09-20
Date Acceptance
2001-08-01
Citation
Nature, 2001, 413 (6853), pp.307-311
ISSN
0028-0836
Publisher
Nature Research
Start Page
307
End Page
311
Journal / Book Title
Nature
Volume
413
Issue
6853
Copyright Statement
© 2001 Macmillan Magazines Ltd. The final publication is available at Springer via https://doi.org/10.1038/35095061
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000171040500039&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
ARABIDOPSIS ROOT
CELL-DIFFERENTIATION
THALIANA
ORGANIZATION
MERISTEM
MORPHOGENESIS
TRAFFICKING
EXPRESSION
POSITION
PROTEINS
Publication Status
Published
Date Publish Online
2001-09-20