Platinum(II)-based optical probes for imaging quadruplex DNA structures via phosphorescence lifetime imaging microscopy
Author(s)
Berrones Reyes, Jessica
Sherin, Peter S
Sarkar, Amrita
Kuimova, Marina K
Vilar, Ramon
Type
Journal Article
Abstract
G-quadruplex DNA is a non-canonical structure that forms in guanine-rich regions of the genome. There is increasing evidence showing that G-quadruplexes have important biological functions, and therefore molecular tools to visualise these structures are important. Herein we report on a series of new cyclometallated platinum(II) complexes which, upon binding to G-quadruplex DNA, display an increase in their phosphorescence, acting as switch-on probes. More importantly, upon binding to G-quadruplexes they display a selective and distinct lengthening of their emission lifetime. We show that this effect can be used to selectively visualise these structures in cells using Phosphorescence Lifetime Imaging Microscopy (PLIM).
Date Issued
2023-10-16
Date Acceptance
2023-08-01
Citation
Angewandte Chemie, 2023, 135 (42), pp.e202310402-e202310402
ISSN
0044-8249
Publisher
Wiley
Start Page
e202310402
End Page
e202310402
Journal / Book Title
Angewandte Chemie
Volume
135
Issue
42
Copyright Statement
© 2023 The Authors. Angewandte Chemie published by Wiley-VCH GmbH
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/38516271
Subjects
Cellular Imaging
Phosphorescence
Platinum
PLIM
Quadruplex DNA
Publication Status
Published
Coverage Spatial
Germany
Article Number
e202310402
Date Publish Online
2023-08-29