Bacteriophage mediates efficient gene transfer in combination with conventional transfection reagents
File(s)
Published version
Accepted version
Author(s)
Donnelly, A
Yata, T
Bentayebi, K
Suwan, K
Hajitou, A
Type
Journal Article
Abstract
The development of commercially available transfection reagents for gene transfer applications has revolutionized the field of molecular biology and scientific research. However, the challenge remains in ensuring that they are efficient, safe, reproducible and cost effective. Bacteriophage (phage)-based viral vectors have the potential to be utilized for general gene transfer applications within research and industry. Yet, they require adaptations in order to enable them to efficiently enter cells and overcome mammalian cellular barriers, as they infect bacteria only; furthermore, limited progress has been made at increasing their efficiency. The production of a novel hybrid nanocomplex system consisting of two different nanomaterial systems, phage vectors and conventional transfection reagents, could overcome these limitations. Here we demonstrate that the combination of cationic lipids, cationic polymers or calcium phosphate with M13 bacteriophage-derived vectors, engineered to carry a mammalian transgene cassette, resulted in increased cellular attachment, entry and improved transgene expression in human cells. Moreover, addition of a targeting ligand into the nanocomplex system, through genetic engineering of the phage capsid further increased gene expression and was effective in a stable cell line generation application. Overall, this new hybrid nanocomplex system i) provides enhanced phage-mediated gene transfer, ii) is applicable for laboratory transfection processes and iii) shows promise within industry for large-scale gene transfer applications.
Date Issued
2015-12-08
Date Acceptance
2015-12-01
Citation
Viruses-Basel, 2015, 7 (12), pp.6476-6489
ISSN
1999-4915
Publisher
MDPI
Start Page
6476
End Page
6489
Journal / Book Title
Viruses-Basel
Volume
7
Issue
12
Copyright Statement
© 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open
access article distributed under the terms and conditions of the Creative Commons by
Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
access article distributed under the terms and conditions of the Creative Commons by
Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
License URL
Sponsor
Medical Research Council (MRC)
Grant Number
G0701159
Subjects
Bacteriophage
calcium phosphate
phage biotechnology
phage-based gene delivery
transfection
Publication Status
Published