Shotgun Lipidomic Profiling of the NCI60 Cell Line Panel Using Rapid Evaporative Ionization Mass Spectrometry.
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
Rapid evaporative ionization mass spectrometry (REIMS) was used for the rapid mass spectrometric profiling of cancer cell lines. Spectral reproducibility was assessed for three different cell lines, and the extent of interclass differences and intraclass variance was found to allow the identification of these cell lines based on the REIMS data. Subsequently, the NCI60 cell line panel was subjected to REIMS analysis, and the resulting data set was investigated for its distinction of individual cell lines and different tissue types of origin. Information content of REIMS spectral profiles of cell lines were found to be similar to those obtained from mammalian tissues although pronounced differences in relative lipid intensity were observed. Ultimately, REIMS was shown to detect changes in lipid content of cell lines due to mycoplasma infection. The data show that REIMS is an attractive means to study cell lines involving minimal sample preparation and analysis times in the range of seconds.
Date Issued
2016-07-05
Date Acceptance
2016-07-05
Citation
Analytical Chemistry, 2016, 88 (15), pp.7507-7514
ISSN
1086-4377
Publisher
American Chemical Society
Start Page
7507
End Page
7514
Journal / Book Title
Analytical Chemistry
Volume
88
Issue
15
Copyright Statement
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://dx.doi.org/10.1021/acs.analchem.6b00187
Subjects
Science & Technology
Physical Sciences
Chemistry, Analytical
Chemistry
DESORPTION ELECTROSPRAY-IONIZATION
BREAST-CANCER
IN-VIVO
IDENTIFICATION
MICROORGANISMS
DISCOVERY
MELANOMA
DATABASE
Analytical Chemistry
0301 Analytical Chemistry
0904 Chemical Engineering
0399 Other Chemical Sciences
Publication Status
Published