Platelets augment respiratory burst in neutrophils activated by selected species of gram-positive or gram-negative bacteria
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
: Neutrophils and platelets circulate in blood system and play important physiological roles as part of immunological
system. Neutrophils are the first line of host defense against various intruders, and platelets are satellite cells cooperating
with other components of defense system. Recent studies report about the cooperation among these types of cells.
We analyzed the effect of platelets on oxygen burst in neutrophils triggered by Staphylococcus aureus and Escherichia coli
bacteria in vitro. The effect of platelets on oxygen burst in neutrophils was measured by luminol enhanced chemiluminescence.
Opsonized and non-opsonized bacteria were used as activators. Activation of neutrophils with live non-opsonized and
opsonized bacteria in the presence of platelets increased the oxygen burst as compared to the same system without platelets.
The gram-positive bacteria (Staphylococcus aureus) were causing higher activation than gram-negative bacteria
(Escherichia coli). This work demonstrate that platelets potentate the response of neutrophils augmenting their respiratory
burst in vitro when triggered by bacteria.
system. Neutrophils are the first line of host defense against various intruders, and platelets are satellite cells cooperating
with other components of defense system. Recent studies report about the cooperation among these types of cells.
We analyzed the effect of platelets on oxygen burst in neutrophils triggered by Staphylococcus aureus and Escherichia coli
bacteria in vitro. The effect of platelets on oxygen burst in neutrophils was measured by luminol enhanced chemiluminescence.
Opsonized and non-opsonized bacteria were used as activators. Activation of neutrophils with live non-opsonized and
opsonized bacteria in the presence of platelets increased the oxygen burst as compared to the same system without platelets.
The gram-positive bacteria (Staphylococcus aureus) were causing higher activation than gram-negative bacteria
(Escherichia coli). This work demonstrate that platelets potentate the response of neutrophils augmenting their respiratory
burst in vitro when triggered by bacteria.
Date Issued
2008-12-06
Date Acceptance
2008-06-01
Citation
Folia Histochemica et Cytobiologica, 2008, 46 (3), pp.383-388
ISSN
1897-5631
Publisher
Polskiego Towarzystwa Histochemików i Cytochemików (Polish Histochemical and Cytochemical Society)
Start Page
383
End Page
388
Journal / Book Title
Folia Histochemica et Cytobiologica
Volume
46
Issue
3
Copyright Statement
© Polish Histochemical et Cytochemical Society 2008.
Subjects
Oncology & Carcinogenesis
Biochemistry And Cell Biology
Publication Status
Published