Age-related alterations in blood and colonic dendritic cell properties
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Background Dendritic cells (DC) determine initiation, type and location of immune
responses and, in adults, show decreased Toll-like receptors and some increased
cytokine levels on ageing. Few studies in children have characterised DC or
explored DC-related mechanisms producing age-related immune changes.
Methods Blood and colonic DC phenotypes were determined in healthy adults and
children by flow cytometry and correlated with aging. Blood DC were divided into
plasmacytoid (pDC) and myeloid (mDC) while only mDC were identified in colon.
Serum cytokine levels were determined by multiplex cytokine assays and correlated
with DC properties.
Results The pDC marker BDCA2 (but not CD123) was absent in pre-pubertal
children and numbers of pDC decreased with age. Blood and colonic DC were more
mature and activated in adults. Decrease in pDC numbers correlated with reduced
GM-CSF levels with aging, but increasing IL-4 and IL-8 levels correlated with a more
activated DC profile in blood. CXCL16 levels decreased with age.
Conclusions In children, lack of BDCA2, a receptor mediating antigen capture and
inhibiting interferon induction, may be immunologically beneficial during immune
development. Conversely, reduced pDC numbers, probably secondary to
decreasing GM-CSF and increasing cytokine-induced maturation of DC are likely to
determine deteriorating immunity with ageing.
responses and, in adults, show decreased Toll-like receptors and some increased
cytokine levels on ageing. Few studies in children have characterised DC or
explored DC-related mechanisms producing age-related immune changes.
Methods Blood and colonic DC phenotypes were determined in healthy adults and
children by flow cytometry and correlated with aging. Blood DC were divided into
plasmacytoid (pDC) and myeloid (mDC) while only mDC were identified in colon.
Serum cytokine levels were determined by multiplex cytokine assays and correlated
with DC properties.
Results The pDC marker BDCA2 (but not CD123) was absent in pre-pubertal
children and numbers of pDC decreased with age. Blood and colonic DC were more
mature and activated in adults. Decrease in pDC numbers correlated with reduced
GM-CSF levels with aging, but increasing IL-4 and IL-8 levels correlated with a more
activated DC profile in blood. CXCL16 levels decreased with age.
Conclusions In children, lack of BDCA2, a receptor mediating antigen capture and
inhibiting interferon induction, may be immunologically beneficial during immune
development. Conversely, reduced pDC numbers, probably secondary to
decreasing GM-CSF and increasing cytokine-induced maturation of DC are likely to
determine deteriorating immunity with ageing.
Date Issued
2016-03-01
Date Acceptance
2015-11-24
Citation
Oncotarget, 2016, 7 (11), pp.11913-11922
ISSN
1949-2553
Publisher
Impact Journals
Start Page
11913
End Page
11922
Journal / Book Title
Oncotarget
Volume
7
Issue
11
Copyright Statement
© 2016 The Authors. Licensed under a Creative Commons Attribution 3.0 License.
License URL
Sponsor
Biotechnology and Biological Sciences Research Cou
Biotechnology and Biological Sciences Research Council
Grant Number
PR5858-000-A
PR5858-000-A
Subjects
BDCA-2
Gerotarget
aging
children
cytokines
mDC
pDC
Publication Status
Published