Comment on “Atlas of fshr expression from novel reporter mice”
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
In recent years, there have been controversial claims of extragonadal effects of follicle-stimulating hormone (FSH). A paper by Chen et al (1) recently published in eLife further muddies the waters, though its intent was to provide clarity. Chen et al developed a new transgenic reporter mouse designed to map FSH receptor (FSHR) expression throughout the body. Using CRISPR/Cas9, the authors reportedly inserted a DNA fragment encoding a P2A-ZsGreen fluorescent protein between the end of the Fshr coding sequence and its 3′ untranslated region. These mice were expected to express a bicistronic Fshr/ZsGreen transcript under the control of the endogenous Fshr regulatory sequences. If successful, FSHR and ZsGreen should be coexpressed in cells as two distinct proteins via ribosomal skipping driven by the P2A sequence, with the former faithfully reporting the endogenous expression of the latter. The ZsGreen signal was unexpectedly observed in nearly all extragonadal tissues and cells examined. Even within the gonads, the reporter was more abundant in noncanonical FSH targets than in Sertoli or granulosa cells.
Date Issued
2025-05-01
Date Acceptance
2025-03-26
Citation
Endocrinology, 2025, 166 (5)
ISSN
0013-7227
Publisher
Oxford University Press
Journal / Book Title
Endocrinology
Volume
166
Issue
5
Copyright Statement
© The Author(s) 2025. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. This is the author’s accepted manuscript made available under a CC-BY licence in accordance with Imperial’s Research Publications Open Access policy (www.imperial.ac.uk/oa-policy)
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/40179242
PII: 8105647
Subjects
FSHR
Sertoli
extragonadal
granulosa
reproduction
Publication Status
Published
Coverage Spatial
United States
Article Number
bqaf066
Date Publish Online
2025-04-03