Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Pathogens modulate plant cell structure and function by secreting effectors into host tissues. Effectors typically function by associating with host molecules and modulating their activities. This study aimed to identify the host processes targeted by the RXLR class of host-translocated effectors of the potato blight pathogen Phytophthora infestans. To this end, we performed an in planta protein-protein interaction screen by transiently expressing P. infestans RXLR effectors in Nicotiana benthamiana leaves followed by co-immunoprecipitation and liquid chromatography tandem mass spectrometry. This screen generated an effector-host protein interactome matrix of 59 P. infestans RXLR effectors x 586 N. benthamiana proteins. Classification of the host interactors into putative functional categories revealed over 35 biological processes possibly targeted by P. infestans. We further characterized the PexRD12/31 family of RXLR-WY effectors, which associate and co-localize with components of the vesicle trafficking machinery. One member of this family, PexRD31, increased the number of FYVE positive vesicles in N. benthamiana cells. FYVE positive vesicles also accumulated in leaf cells near P. infestans hyphae, indicating that the pathogen may enhance endosomal trafficking during infection. This interactome data set will serve as a useful resource for functional studies of P. infestans effectors and of effector-targeted host processes.
Date Issued
2021-05-01
Date Acceptance
2021-02-19
Citation
The Plant Cell, 2021, 33 (5), pp.1447-1471
ISSN
1040-4651
Publisher
American Society of Plant Biologists
Start Page
1447
End Page
1471
Journal / Book Title
The Plant Cell
Volume
33
Issue
5
Copyright Statement
©The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution,and reproduction in any medium, provided the original work is properly cited
License URL
Sponsor
Biotechnology and Biological Sciences Research Council (BBSRC)
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/33677602
PII: 6156641
Grant Number
BB/T006102/1
Subjects
Plant Biology & Botany
0601 Biochemistry and Cell Biology
0604 Genetics
0607 Plant Biology
Publication Status
Published
Coverage Spatial
England
Date Publish Online
2021-03-02