Background: GPCRs form complex oligomers whose role in signaling is poorly understood.
Results: Super-resolution imaging of functionally asymmetric oligomers reveals diverse functional and structural organizations
and the ability to alter signal responses.
Conclusion: GPCR oligomers may fine-tune receptor signaling by altering the functional role of individual protomers.
Significance: Distinct oligomers could be exploited pharmacologically to improve efficacy, selectivity, and/or specificity.