Mapping the metabolome in the developing gut
File(s)
Author(s)
Sani, Maria
Type
Thesis
Abstract
The functional evolution of the gut metabolome in neonatology, especially in premature infants, is not fully understood. This study investigates the relationship between the gut microbiome and metabolome in early development using advanced analytical chemistry techniques. The study utilizes untargeted RP LC-MS based lipidomics and targeted LC-MS assays to analyse neonate faecal samples for lipid and tryptophan metabolism, including in the context of NEC. Several significant pairwise interactions between factors like birthweight, infections, mode of delivery, and chorioamnionitis were identified, highlighting potential lipid biomarkers. The study also explores the association between the gut metabolome and eczema, as well as its role in NEC pathogenesis and prognosis. In a pilot study, fatty acyls, glycolipids, and sphingolipids were found to be elevated in NEC cases, suggesting their involvement in NEC pathogenesis. The targeted method identified specific metabolite differences in NEC diagnosis, emphasizing the potential role of certain compounds in the disease. The study emphasizes the significance of lipid metabolism and its impact on various health conditions in neonates. The untargeted lipidomics assay shows promise as a predictive tool for eczema development. Overall, this research provides valuable insights into the gut metabolome in neonatology, informing our understanding of the complex interplay between the gut microbiome and metabolome in early development and its implications for neonatal health.
Version
Open Access
Date Issued
2023-11-27
Date Awarded
2024-06-01
Copyright Statement
Attribution-NonCommercial 4.0 International Licence (CC BY-NC)
License URL
Advisor
Kinross, James
Takats, Zoltan
Marchesi, Julian
Publisher Department
Department of Surgery & Cancer
Publisher Institution
Imperial College London
Qualification Level
Doctoral
Qualification Name
Doctor of Philosophy (PhD)