A Molecular Rotor that Measures Dynamic Changes of Lipid Bilayer Viscosity Caused by Oxidative Stress
File(s)
Supporting information
Published version
Author(s)
Vysniauskas, A
Qurashi, M
Kuimova, MK
Type
Journal Article
Abstract
Oxidation of cellular structures is typically an undesirable process that can be a hallmark of certain diseases. On the other hand, photooxidation is a necessary step of photodynamic therapy (PDT), a cancer treatment causing cell death upon light irradiation. Here, the effect of photooxidation on the microscopic viscosity of model lipid bilayers constructed of 1,2-dioleoyl-sn-glycero-3-phosphocholine has been studied. A molecular rotor has been employed that displays a viscosity-dependent fluorescence lifetime as a quantitative probe of the bilayer's viscosity. Thus, spatially-resolved viscosity maps of lipid photooxidation in giant unilamellar vesicles (GUVs) were obtained, testing the effect of the positioning of the oxidant relative to the rotor in the bilayer. It was found that PDT has a strong impact on viscoelastic properties of lipid bilayers, which ‘travels’ through the bilayer to areas that have not been irradiated directly. A dramatic difference in viscoelastic properties of oxidized GUVs by Type I (electron transfer) and Type II (singlet oxygen-based) photosensitisers was also detected.
Date Issued
2016-08-03
Date Acceptance
2016-06-14
Citation
Chemistry-A European Journal, 2016, 22 (37), pp.13210-13217
ISSN
1521-3765
Publisher
Wiley
Start Page
13210
End Page
13217
Journal / Book Title
Chemistry-A European Journal
Volume
22
Issue
37
Copyright Statement
© 2016 The Authors. This is an open access article under the terms of the Creative Commons At-tribution License, which permits use, distribution and reproduction in anymedium, provided the original work is properly cited
License URL
Sponsor
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Grant Number
EP/I003983/1
EP/K503381/1
EP/K030760/1
Subjects
fluorescent probes
lipid oxidation
liposomes
microviscosity
molecular rotors
General Chemistry
03 Chemical Sciences
Publication Status
Published