Förster resonance energy transfer imaging in vivo with approximated radiative transfer equation
File(s)
Accepted version
OA Location
Author(s)
Type
Journal Article
Abstract
We describe a new light transport model, which was applied to three-dimensional lifetime imaging of Förster resonance energy transfer in mice in vivo. The model is an approximation to the radiative transfer equation and combines light diffusion and ray optics. This approximation is well adopted to wide-field time-gated intensity-based data acquisition. Reconstructed image data are presented and compared with results obtained by using the telegraph equation approximation. The new approach provides improved recovery of absorption and scattering parameters while returning similar values for the fluorescence parameters.
Date Issued
2011-12-15
Citation
Applied Optics, 2011, 50 (36), pp.6583-6590
Start Page
6583
End Page
6590
Journal / Book Title
Applied Optics
Volume
50
Issue
36
Copyright Statement
© 2011 Optical Society of America
Subjects
Science & Technology
Physical Sciences
Optics
OPTICS
FLUORESCENCE LIFETIME TOMOGRAPHY
HIGHLY SCATTERING INCLUSIONS
OPTICAL TOMOGRAPHY
TURBID MEDIA
TRANSFER PARAMETERS
Algorithms
Animals
Fluorescence
Fluorescence Resonance Energy Transfer
Imaging, Three-Dimensional
Mice
Models, Theoretical
Optics and Photonics
Scattering, Radiation
Tomography, Optical
0205 Optical Physics
0913 Mechanical Engineering
0906 Electrical And Electronic Engineering