Simultaneous monitoring of tyrosinase and ATP in thick brain tissues using a single two‐photon fluorescent probe
Author(s)
Type
Journal Article
Abstract
Cellular redox homeostasis and energy metabolism in the central nervous system are associated with neurodegenerative diseases. However, their real-time and concurrent monitoring in thick tissues remains challenging. Herein, a single dual-emission two-photon fluorescent probe (named DST) is designed for the simultaneous tracking of tyrosinase (TYR) and adenosine triphosphate (ATP), thereby enabling the real-time monitoring of both neurocellular redox homeostasis and energy metabolism in brain tissue. The developed DST probe exhibits excellent sensitivity and selectivity toward TYR and ATP, with distinctive responses in the blue and red fluorescence channels being observed without spectra crosstalk. Using this probe, the correlation and regulatory mechanism between TYR and ATP during oxidative stress are uncovered. Additionally, the two-photon nature of this probe allows alterations in the TYR and ATP levels to be monitored across different brain regions in an Alzheimer's disease (AD) mouse model. Notably, a significant decrease in ATP levels is revealed within the somatosensory cortex (S1BF) and caudate putamen brain regions of an AD mouse, alongside an increase in TYR levels within the S1BF and laterodorsal thalamic nucleus brain regions. These findings indicate the potential of applying the spatially resolved regulation of neurocellular redox homeostasis and energy metabolism to treat neurodegenerative diseases.
Date Issued
2025-05-22
Date Acceptance
2025-03-01
Citation
Advanced Science, 2025, 12 (19)
ISSN
2198-3844
Publisher
Wiley
Journal / Book Title
Advanced Science
Volume
12
Issue
19
Copyright Statement
© 2025 The Author(s). Advanced Science published by Wiley-VCH GmbH This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/40129186
Subjects
ATP
alzheimer's disease
brain tissues
two‐photon imaging
tyrosinase
Publication Status
Published
Coverage Spatial
Germany
Article Number
2413220
Date Publish Online
2025-03-24