The Functional Study of the N-Terminal Region of Influenza B Virus Nucleoprotein
File(s)
Author(s)
Type
Journal Article
Abstract
Influenza nucleoprotein (NP) is a major component of the ribonucleoprotein (vRNP) in influenza
virus, which functions for the transcription and replication of viral genome. Compared
to the nucleoprotein of influenza A (ANP), the N-terminal region of influenza B nucleoprotein
(BNP) is much extended. By virus reconstitution, we found that the first 38 residues are
essential for viral growth. We further illustrated the function of BNP by mini-genome reconstitution,
fluorescence microscopy, electron microscopy, light scattering and gel shift.
Results show that the N terminus is involved in the formation of both higher homo-oligomers
of BNP and BNP-RNA complex.
virus, which functions for the transcription and replication of viral genome. Compared
to the nucleoprotein of influenza A (ANP), the N-terminal region of influenza B nucleoprotein
(BNP) is much extended. By virus reconstitution, we found that the first 38 residues are
essential for viral growth. We further illustrated the function of BNP by mini-genome reconstitution,
fluorescence microscopy, electron microscopy, light scattering and gel shift.
Results show that the N terminus is involved in the formation of both higher homo-oligomers
of BNP and BNP-RNA complex.
Date Issued
2015-09-14
Date Acceptance
2015-08-21
Citation
PLOS One, 2015, 10 (9)
ISSN
1932-6203
Publisher
Public Library of Science
Journal / Book Title
PLOS One
Volume
10
Issue
9
Copyright Statement
© 2015 Liu et al. This is an open access
article distributed under the terms of the Creative
Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any
medium, provided the original author and source are
credited
article distributed under the terms of the Creative
Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any
medium, provided the original author and source are
credited
License URL
Sponsor
Crucell Holland B.V.
Grant Number
FLU-M-D1000NL1
Subjects
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
VESICULAR STOMATITIS-VIRUS
NUCLEAR-LOCALIZATION
RNA-BINDING
NEURAMINIDASE INHIBITORS
REDUCED SENSITIVITY
MODEL RNA
REPLICATION
PROTEIN
COMPLEX
ENCAPSIDATION
Publication Status
Published
Article Number
e0137802