Probing bacterial-fungal interactions at the single cell level.
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
Interactions between fungi and prokaryotes are abundant in many ecological systems. A wide variety of biomolecules regulate such interactions and many of them have found medicinal or biotechnological applications. However, studying a fungal-bacterial system at a cellular level is technically challenging. New microfluidic devices provided a platform for microscopic studies and for long-term, time-lapse experiments. Application of these novel tools revealed insights into the dynamic interactions between the basidiomycete Coprinopsis cinerea and the bacterium Bacillus subtilis. Direct contact was mediated by polar attachment of bacteria to only a subset of fungal hyphae suggesting a differential competence of fungal hyphae and thus differentiation of hyphae within a mycelium. The fungicidal activity of B. subtilis was monitored at a cellular level and showed a novel mode of action on fungal hyphae.
Date Issued
2014-10
Date Acceptance
2014-08-09
Citation
Integrative Biology: interdisciplinary approaches for molecular and cellular life sciences, 2014, 6 (10), pp.935-945
ISSN
1757-9694
Publisher
Oxford University Press (OUP)
Start Page
935
End Page
945
Journal / Book Title
Integrative Biology: interdisciplinary approaches for molecular and cellular life sciences
Volume
6
Issue
10
Copyright Statement
© The Royal Society of Chemistry 2014. This is a pre-copy-editing, author-produced version of an article accepted for publication in Integrative Biology following peer review. The definitive publisher-authenticated version Claire E. Stanley, Martina Stöckli, Dirk van Swaay, Jerica Sabotič, Pauli T. Kallio, Markus Künzler, Andrew J. deMello, Markus Aebi, Probing bacterial–fungal interactions at the single cell level, Integrative Biology, Volume 6, Issue 10, October 2014, Pages 935–945 is available online at: https://doi.org/10.1039/c4ib00154k
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/25144657
Subjects
Bacillus subtilis
Basidiomycota
Hyphae
Microfluidics
Microscopy, Fluorescence
Time-Lapse Imaging
Publication Status
Published
Coverage Spatial
England
Date Publish Online
2014-08-21