Exploring complex ecological patterns in hyper-diverse tropical canopy arthropod communities using modern genetic technologies
File(s)
Author(s)
Creedy, Thomas
Type
Thesis
Abstract
The canopy arthropod community in tropical montane cloud forests has rarely achieved research attention, but this remote, diverse community is likely under considerable threat from climate change. Modern techniques are synthesised in this thesis to investigate this community. Accurate climate knowledge is necessary for any climate change research, and this thesis utilises current and predicted climate data to aid in sampling, and validates these data using ground-truthed measurements. Modern molecular ecology techniques enable high-taxonomic-resolution studies such as this, but the pipelines need careful validation: this thesis presents an empirical study to examine biases in metabarcoding, new tools for easy validation of bioinformatic pipelines, and discusses taxonomic assignment of anonymous sequences. A new canopy sampling design is employed to sample canopy arthropods in two tree species in a rainforest, and metabarcoding is successfuly used to uncover high levels of spatial turnover and phylobetadiversity across a highly heterogenous landscape in a spatially explicit nested study.
Version
Open Access
Date Issued
2017-09
Date Awarded
2018-05
Copyright Statement
Attribution NoDerivatives 4.0 International Licence (CC BY-ND)
Advisor
Vogler, Alfried
Kovac, Mirko
Sponsor
Imperial College London
Publisher Department
Life Sciences
Publisher Institution
Imperial College London
Qualification Level
Doctoral
Qualification Name
Doctor of Philosophy (PhD)