Detection of glial fibrillary acidic protein in patient plasma using on-chip graphene field-effect biosensors, in comparison with ELISA and single molecule array
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Glial fibrillary acidic protein (GFAP) is a discriminative blood biomarker for many neurological diseases, such as traumatic brain injury. Detection of GFAP in buffer solutions using biosensors has been demonstrated, but accurate quantification of GFAP in patient samples has not been reported, yet in urgent need. Herein, we demonstrate a robust on-chip graphene field-effect transistor (GFET) biosensing method for sensitive and ultrafast detection of GFAP in patient plasma. Patients with moderate–severe traumatic brain injuries, defined by the Mayo classification, are recruited to provide plasma samples. The binding of target GFAP with the specific antibodies that are conjugated on a monolayer GFET device triggers the shift of its Dirac point, and this signal change is correlated with the GFAP concentration in the patient plasma. The limit of detection (LOD) values of 20 fg/mL (400 aM) in buffer solution and 231 fg/mL (4 fM) in patient plasma have been achieved using this approach. In parallel, for the first time, we compare our results to the state-of-the-art single-molecule array (Simoa) technology and the classic enzyme-linked immunosorbent assay (ELISA) for reference. The GFET biosensor shows competitive LOD to Simoa (1.18 pg/mL) and faster sample-to-result time (<15 min), and also it is cheaper and more user-friendly. In comparison to ELISA, GFET offers advantages of total detection time, detection sensitivity, and simplicity. This GFET biosensing platform holds high promise for the point-of-care diagnosis and monitoring of traumatic brain injury in GP surgeries and patient homes.
Date Issued
2021-12-15
Date Acceptance
2021-12-07
Citation
ACS Sensors, 2021, 7 (1), pp.253-262
ISSN
2379-3694
Publisher
American Chemical Society
Start Page
253
End Page
262
Journal / Book Title
ACS Sensors
Volume
7
Issue
1
Copyright Statement
© 2021 The Authors. Published by American Chemical Society. This article is available open access under a CC-BY Licence (https://creativecommons.org/licenses/by/4.0/)
License URL
Sponsor
UK DRI Ltd
Identifier
https://pubs.acs.org/doi/10.1021/acssensors.1c02232
Grant Number
N/A
Subjects
Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry, Analytical
Nanoscience & Nanotechnology
Chemistry
Science & Technology - Other Topics
single-molecule array
graphene fi eld-e ff ect transistor
biosensor
glial fi brillary acidic protein
DIAGNOSTIC MARKER
GFAP
BIOMARKERS
biosensor
glial fibrillary acidic protein
single-molecule array, graphene field-effect transistor
traumatic brain injury blood biomarker
0301 Analytical Chemistry
0903 Biomedical Engineering
1007 Nanotechnology
Publication Status
Published
Date Publish Online
2021-12-15