Variation of volatile organic compound levels within ambient room air and its impact upon the standardisation of breath sampling
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Published version
Author(s)
Hewitt, Michael
Belluomo, Ilaria
Zuffa, Simone
Boshier, Piers
Myridakis, Antonios
Type
Journal Article
Abstract
INTRODUCTION: The interest around analysis of volatile organic compounds (VOCs) within breath has increased in the last two decades. Uncertainty remains around standardisation of sampling and whether VOCs within room air can influence breath VOC profiles.
OBJECTIVES: To assess the abundance of VOCs within room air in common breath sampling locations within a hospital setting and whether this influences the composition of breath. A secondary objective is to investigate diurnal variation in room air VOCs.
METHODS: Room air was collected using a sampling pump and thermal desorption (TD) tubes in the morning and afternoon from five locations. Breath samples were collected in the morning only. TD tubes were analysed using gas chromatography coupled with time-of-flight mass spectrometry (GC-TOF-MS).
RESULTS: A total of 113 VOCs were identified from the collected samples. Multivariate analysis demonstrated clear separation between breath and room air. Room air composition changed throughout the day and different locations were characterized by specific VOCs, which were not influencing breath profiles.
CONCLUSIONS: Breath did not demonstrate separation based on location, suggesting that sampling can be performed across different locations without affecting results.
OBJECTIVES: To assess the abundance of VOCs within room air in common breath sampling locations within a hospital setting and whether this influences the composition of breath. A secondary objective is to investigate diurnal variation in room air VOCs.
METHODS: Room air was collected using a sampling pump and thermal desorption (TD) tubes in the morning and afternoon from five locations. Breath samples were collected in the morning only. TD tubes were analysed using gas chromatography coupled with time-of-flight mass spectrometry (GC-TOF-MS).
RESULTS: A total of 113 VOCs were identified from the collected samples. Multivariate analysis demonstrated clear separation between breath and room air. Room air composition changed throughout the day and different locations were characterized by specific VOCs, which were not influencing breath profiles.
CONCLUSIONS: Breath did not demonstrate separation based on location, suggesting that sampling can be performed across different locations without affecting results.
Date Issued
2022-09-23
Date Acceptance
2022-09-12
Citation
Scientific Reports, 2022, 12, pp.1-7
ISSN
2045-2322
Publisher
Nature Publishing Group
Start Page
1
End Page
7
Journal / Book Title
Scientific Reports
Volume
12
Copyright Statement
© The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
License URL
Identifier
https://www.nature.com/articles/s41598-022-20365-7
Subjects
Air
Air Pollutants
Body Fluids
Breath Tests
Gas Chromatography-Mass Spectrometry
Volatile Organic Compounds
Body Fluids
Air Pollutants
Breath Tests
Air
Gas Chromatography-Mass Spectrometry
Volatile Organic Compounds
Publication Status
Published
Date Publish Online
2022-09-23