Plasmonic nanoprobes for stimulated emission depletion nanoscopy
File(s)SI_Cortes et al_final.docx (3.69 MB) MS_Cortes et al.docx (2.42 MB)
Supporting information
Accepted version
Author(s)
Type
Journal Article
Abstract
Plasmonic nanoparticles influence the absorption and emission processes of nearby emitters due to local enhancements of the illuminating radiation and the photonic density of states. Here, we use the plasmon resonance of metal nanoparticles in order to enhance the stimulated depletion of excited molecules for super-resolved nanoscopy. We demonstrate stimulated emission depletion (STED) nanoscopy with gold nanorods with a long axis of only 26 nm and a width of 8 nm. These particles provide an enhancement of up to 50% of the resolution compared to fluorescent-only probes without plasmonic components irradiated with the same depletion power. The nanoparticle-assisted STED probes reported here represent a ∼2 × 103 reduction in probe volume compared to previously used nanoparticles. Finally, we demonstrate their application toward plasmon-assisted STED cellular imaging at low-depletion powers, and we also discuss their current limitations.
Date Issued
2016-10-30
Date Acceptance
2016-10-30
Citation
ACS Nano, 2016, 10 (11), pp.10454-10461
ISSN
1936-0851
Publisher
American Chemical Society
Start Page
10454
End Page
10461
Journal / Book Title
ACS Nano
Volume
10
Issue
11
Copyright Statement
© 2016 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Nano, after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acsnano.6b06361.
Sponsor
The Royal Society
The Leverhulme Trust
Engineering & Physical Science Research Council (E
Grant Number
WM110079
RPG-2013-119
EP/M013812/1
Subjects
STED nanoscopy
bioimaging
nanorods
plasmonic nanoparticles
super resolution
Nanoscience & Nanotechnology
MD Multidisciplinary
Publication Status
Published