Enhanced raman investigation of cell membrane and intracellular compounds by 3D plasmonic nanoelectrode arrays
Author(s)
Type
Journal Article
Abstract
3D nanostructures are widely exploited in cell cultures for many purposes such as controlled drug delivery, transfection, intracellular sampling, and electrical recording. However, little is known about the interaction of the cells with these substrates, and even less about the effects of electroporation on the cellular membrane and the nuclear envelope. This work exploits 3D plasmonic nanoelectrodes to study, by surface‐enhanced Raman scattering (SERS), the cell membrane dynamics on the nanostructured substrate before, during, and after electroporation. In vitro cultured cells tightly adhere on 3D plasmonic nanoelectrodes precisely in the plasmonic hot spots, making this kind of investigation possible. After electroporation, the cell membrane dynamics are studied by recording the Raman time traces of biomolecules in contact or next to the 3D plasmonic nanoelectrode. During this process, the 3D plasmonic nanoelectrodes are intracellularly coupled, thus enabling the monitoring of different molecular species, including lipids, proteins, and nucleic acids. Scanning electron microscopy cross‐section analysis evidences the possibility of nuclear membrane poration compatible with the reported Raman spectra. These findings may open a new route toward controlled intracellular sampling and intranuclear delivery of genic materials. They also show the possibility of nuclear envelope disruption which may lead to negative side effects.
Date Issued
2018-12-01
Date Acceptance
2018-07-31
Citation
Advanced Science, 2018, 5 (12)
ISSN
2198-3844
Publisher
Wiley Open Access
Journal / Book Title
Advanced Science
Volume
5
Issue
12
Copyright Statement
© 2018 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000453685900008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Physical Sciences
Technology
Chemistry, Multidisciplinary
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Chemistry
Science & Technology - Other Topics
Materials Science
electroporation
intracellular spectroscopy
microelectrode arrays
nanopillars
permeabilization
Raman spectroscopy
ACTION-POTENTIALS
IN-VITRO
SPECTROSCOPY
ELECTROPORATION
DELIVERY
ELECTROPERMEABILIZATION
NANOPARTICLES
NANONEEDLES
RECORDINGS
PLATFORM
Publication Status
Published
Article Number
1800560
Date Publish Online
2018-10-23