Development and workflow of a continuous protein crystallization process: a case of lysozyme
File(s)
Accepted version
Author(s)
Yang, Huaiyu
Chen, Wenqian
Peczulis, Peter
Heng, Jerry YY
Type
Journal Article
Abstract
In the present work, a workflow on the development of a continuous protein crystallisation is introduced, with lysozyme as a model protein, from micro L screening experiments, to small scale batch crystallisation experiments in a shaking crystallisation platform, and to batch and continuous crystallisation experiments in an oscillatory flow platform. The lysozyme crystallisation investigated were for a concentration range from 30 to 100 mg/mL, shaking conditions from 100 to 200 rpm in the batch shaking crystallisation platform, and oscillatory conditions with amplitude (x) from 5 to 30 mm and frequency (f) from 0.1 to 1.0 Hz in the batch oscillatory flow crystallisation platform. We propose the use of the Reynold’s number (Re) for scaling up of the process from the shaking batch to the continuous oscillatory flow platform. Additionally, it is shown that the nucleation rate increased with increase in concentration of initial lysozyme solution, or increase in shear rate, inducing smaller size of lysozyme crystals. These indicate that continuous crystallisation platforms may offer advantages to the downstream bioprocessing of proteins.
Date Issued
2019-02-06
Date Acceptance
2019-01-01
Citation
Crystal Growth and Design, 2019, 19 (2), pp.983-991
ISSN
1528-7483
Publisher
American Chemical Society
Start Page
983
End Page
991
Journal / Book Title
Crystal Growth and Design
Volume
19
Issue
2
Copyright Statement
© 2019 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Crystal Growth & Design, after peer review and technical editing by the publisher. To access the final edited and published work see https://dx.doi.org/10.1021/acs.cgd.8b01534.
Sponsor
Engineering & Physical Science Research Council (EPSRC)
Identifier
https://pubs.acs.org/doi/10.1021/acs.cgd.8b01534
Grant Number
EP/N015916/1
Subjects
Science & Technology
Physical Sciences
Technology
Chemistry, Multidisciplinary
Crystallography
Materials Science, Multidisciplinary
Chemistry
Materials Science
OSCILLATORY FLOW
BATCH CRYSTALLIZATION
BULK CRYSTALLIZATION
POWER-CONSUMPTION
SCREENING METHOD
CRYSTAL-GROWTH
PURIFICATION
NUCLEATION
SYSTEM
KINETICS
0302 Inorganic Chemistry
0306 Physical Chemistry (incl. Structural)
0912 Materials Engineering
Inorganic & Nuclear Chemistry
Publication Status
Published
Date Publish Online
2019-01-04