Pneumococcal 6-Phospho-β-Glucosidase (BglA3) Is Involved in Virulence and Nutrient Metabolism.
Author(s)
Terra, VS
Zhi, X
Kahya, HF
Andrew, PW
Yesilkaya, H
Type
Journal Article
Abstract
For the generation of energy, the important human pathogen Streptococcus pneumoniae relies on host-derived sugars, including β-glucoside analogs. The catabolism of these nutrients involves the action of 6-phospho-β-glucosidase to convert them into usable monosaccharaides. In this study, we characterized a 6-phospho-β-glucosidase (BglA3) encoded by SPD_0247. We found that this enzyme has a cell membrane localization and is active only against a phosphorylated substrate. A mutated pneumococcal ΔSPD0247 strain had reduced 6-phospho-glucosidase activity and was attenuated in growth on cellobiose and hyaluronic acid compared to the growth of wild-type D39. ΔSPD0247-infected mice survived significantly longer than the wild-type-infected cohort, and the colony counts of the mutant were lower than those of the wild type in the lungs. The expression of SPD_0247 in S. pneumoniae harvested from infected tissues was significantly increased relative to its expression in vitro on glucose. Additionally, ΔSPD0247 is severely impaired in its attachment to an abiotic surface. These results indicate the importance of β-glucoside metabolism in pneumococcal survival and virulence.
Date Issued
2015-12-28
Date Acceptance
2015-10-26
Citation
Infection and Immunity, 2015, 84 (1), pp.286-292
ISSN
0019-9567
Publisher
American Society for Microbiology
Start Page
286
End Page
292
Journal / Book Title
Infection and Immunity
Volume
84
Issue
1
Copyright Statement
Copyright © 2015, American Society for Microbiology. All Rights Reserved.
Identifier
PII: IAI.01108-15
Subjects
Animals
Bacterial Adhesion
Bacterial Proteins
Cellobiose
Energy Metabolism
Female
Glucose
Glucosidases
Hyaluronic Acid
Mice
Phosphorylation
Pneumococcal Infections
Streptococcus pneumoniae
Virulence Factors
Publication Status
Published online
Date Publish Online
2015-12-28