Strontium-containing bioactive glass nanoparticles stimulate osteogenesis and suppress osteoclast formation in co-culture
Author(s)
Naruphontjirakul, Parichart
Porter, Alexandra E
Jones, Julian R
Type
Journal Article
Abstract
The effect of monodispersed bioactive glass nanoparticles containing strontium (9.4 mol % SrO and 4.4 mol% SrO) and their dissolution products on osteoclast differentiation are investigated in monoculture and in an osteoblast-osteoclast in vitro co-culture system. Under standard conditions, RAW264.7 cells efficiently differentiate into osteoclast-like cells upon RANKL stimulation, making them a well-established monoculture model for osteoclastogenesis. The viability of RAW264.7 cells exposed to nanoparticles is dose- and time-dependent. Indirect co-culture of pre-osteoblast MC3T3-E1 and mouse monocyte RAW264.7 cells is carried out using Millipore cell culture plate inserts, with and without RANKL. Osteoclast differentiation, indicated by tartrate-resistant acid phosphatase (TRAP) activity and the formation of multinucleated osteoclasts, is significantly reduced in RAW264.7 cells exposed to Sr-BGNPs and their dissolution products, compared to RANKL-stimulated control cells. Therefore, the observed reduction in osteoclast differentiation following exposure to Sr-BGNPs and their ionic dissolution products indicates a potential inhibitory effect on this process. In co-culture, bioactive glass nanoparticles containing 9.4 mol% SrO2 promoted differentiation of osteoblasts, relative to basal media and osteogenic media controls, measured as an increase in Alkaline phosphotase (ALP) activity and formation of mineralized matrix, while differentiation of osteoclasts decreased, measured as a reduction in TRAP activity and multinucleated osteoclast formation.
Date Issued
2026-02-02
Date Acceptance
2025-10-01
Citation
Advanced healthcare materials, 2026, 15 (5)
ISSN
2192-2640
Publisher
Wiley
Journal / Book Title
Advanced healthcare materials
Volume
15
Issue
5
Copyright Statement
© 2025 The Author(s). Advanced Healthcare Materials published by Wiley-VCH GmbH This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/41137437
Subjects
anti-osteoclastic activity
bioactive glass
BONE-MARROW
DIFFERENTIATION
Engineering
Engineering, Biomedical
indirect co-culture system
Materials Science
Materials Science, Biomaterials
nanoparticles
Nanoscience & Nanotechnology
osteoblast activity
RANKL
RESORPTION
Science & Technology
Science & Technology - Other Topics
SIZE
strontium
Technology
Publication Status
Published
Coverage Spatial
Germany
Article Number
e03671
Date Publish Online
2025-10-24