Identification of missense variants in the C-domains of Von Willebrand Factor that cause gain-of-function-like activity
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Recently characterised mutations Phe2561Tyr, Pro2555Arg in the Von Willebrand Factor (VWF) C4 domain and Gly2705Arg in the C6 domain reportedly confer gain-of-function-like activity on the molecule, increasing responsiveness to shear stress, resulting in enhanced platelet capture. This implies that the C-terminal stem, comprised of the D4-C6 domains, plays a crucial role in modulating VWF function. To further investigate this, we used site-directed mutagenesis to generate a panel of uncharacterised C-domain variants. VWF expression and function were analysed under static and shear stress conditions, and the biophysical properties of the variants were characterised by single-molecule optical tweezers (OT), and atomic force microscopy (AFM) imaging. All the expressed variants exhibited normal function in static assays, except the C1 domain Arg2287Trp variant that demonstrated increased binding to GPIbα. Under flow conditions at 1500s-1, all the variants had normal VWF-mediated platelet capture to collagen, however at 5000s-1 Arg2287Trp demonstrated enhanced platelet capture, while Asn2636Tyr (C5 domain), Thr2647Met (C6 domain) and Gly2705Arg showed reduced activity. Further analysis of the formation of rolling VWF-platelet aggregates over a VWF surface demonstrated an enhanced response to shear stress for the Arg2287Trp and Arg2384Trp (C2 domain) variants. OT analysis identified novel extension events exclusive to the C-terminal domains and more frequent unfolding events and longer unfolding extensions for Arg2287Trp and Arg2384Trp, consistent with increased flexibility and stem opening. AFM imaging confirmed that both variants favoured the open stem conformation. Together, we demonstrate that mutations in the C1 and C2 domains alter stem dynamics, rendering VWF more responsive to shear stress.
Date Issued
2026-06-23
Date Acceptance
2026-02-13
Citation
Blood Advances, 2026, 10 (12), pp.4334-4346
ISSN
2473-9537
Publisher
American Society of Hematology (ASH Publications)
Start Page
4334
End Page
4346
Journal / Book Title
Blood Advances
Volume
10
Issue
12
Copyright Statement
© 2026 American Society of Hematology Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved.
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/41774851
PII: 566912
Publication Status
Published
Coverage Spatial
United States
Date Publish Online
2026-03-03