Calcium- and cell cycle-dependent association of annexin 11 with the nuclear envelope.
File(s)
Published version
OA Location
Author(s)
Tomas, A
Moss, SE
Type
Journal Article
Abstract
Annexin 11 is a widely expressed calcium- and phospholipid-binding protein that resides in the nucleoplasm in many cultured cell lines. This is in contrast to its most extensively characterized in vitro ligand, the small calcium-binding protein S100A6 (calcyclin), which is concentrated in the nuclear envelope. Here we have examined the significance of the association of annexin 11 and S100A6 by asking whether circumstances exist in which the two proteins occupy the same subcellular localization. First, we show that in both A431 and vascular smooth muscle cells, elevation of intracellular Ca2+ leads to translocation of annexin 11 from the nucleus to the nuclear envelope where it co-localizes with S100A6. We also demonstrate, using fusions of annexin 11 with green fluorescent protein, that whereas the C-terminal core domain of annexin 11 is essential for Ca2+ sensitivity, the N-terminal domain is required for targeting to the nuclear envelope. Second, we show that annexin 11 relocalizes to the nuclear envelope as A431 cells transit from early to mid-prophase. In late prophase, at the time of nuclear envelope breakdown, annexin 11 and S100A6 become intensely localized with lamina-associated polypeptide 2 to folds in the nuclear envelope. From metaphase to telophase S100A6 is degraded, but in late telophase annexin 11 associates with the reforming nuclear envelope before resuming a nucleoplasmic location in interphase. These results show that co-localization of annexin 11 and S100A6 at the nuclear envelope may be regulated either by elevation of intracellular Ca2+ or by cell cycle progression and provide the first evidence that these proteins may associate in vivo.
Date Issued
2003-05-30
ISSN
0021-9258
Start Page
20210
End Page
20216
Journal / Book Title
Journal of Biological Chemistry
Volume
278
Issue
22
Copyright Statement
© 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Identifier
http://www.ncbi.nlm.nih.gov/pubmed/12601007
M212669200
Subjects
Annexins
Blotting, Western
Calcium
Cell Cycle
Cell Cycle Proteins
Cell Line
Electrophoresis, Polyacrylamide Gel
Green Fluorescent Proteins
Humans
Luminescent Proteins
Microscopy, Fluorescence
Nuclear Envelope
Precipitin Tests
Recombinant Fusion Proteins
S100 Proteins
Biochemistry & Molecular Biology
06 Biological Sciences
11 Medical And Health Sciences
03 Chemical Sciences
Publication Status
Published
Coverage Spatial
United States