Untargeted LC/MS-based metabolic phenotyping (metabonomics/metabolomics): The state of the art
File(s)
Accepted version
Author(s)
Gika, Helen
Virgiliou, Christina
Theodoridis, Georgios
Plumb, Robert S
Wilson, Ian D
Type
Journal Article
Abstract
Liquid chromatography (LC) hyphenated to mass spectrometry is currently the most widely used means of determining metabolic phenotypes via both untargeted and targeted analysis. At present a range of analytical separations, including reversed-phase, hydrophilic interaction and ion-pair LC are employed to maximise metabolome coverage with ultra (high) performance liquid chromatography (UHPLC) increasingly displacing conventional high performance liquid chromatography because of the need for short analysis times and high peak capacity in such applications. However, it is widely recognized that these methodologies do not entirely solve the problems facing researchers trying to perform comprehensive metabolic phenotyping and in addition to these “routine” approaches there are continuing investigations of alternative separation methods including 2-dimensional/multi column approaches. These involve either new stationary phases or multidimensional combinations of the more conventional materials currently used, as well as application of miniaturization or “new” approaches such as supercritical HP and UHP- chromatographic separations. There is also a considerable amount of interest in the combination of chromatographic and ion mobility separations, with the latter providing both an increase in resolution and the potential to provide additional structural information via the determination of molecular collision cross section data. However, key problems remain to be solved including ensuring quality, comparability across different laboratories and the ever present difficulty of identifying unknowns.
Date Issued
2019-06-01
Date Acceptance
2019-04-03
Citation
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2019, 1117, pp.136-147
ISSN
1570-0232
Publisher
Elsevier
Start Page
136
End Page
147
Journal / Book Title
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume
1117
Copyright Statement
© 2019 Elsevier Ltd. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence http://creativecommons.org/licenses/by-nc-nd/4.0/
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000467891000014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemical Research Methods
Chemistry, Analytical
Biochemistry & Molecular Biology
Chemistry
Metabonomics
Metabolomics
Liquid chromatography
Supercritical fluid chromatography
Ion mobility spectrometry
Mass spectrometry
PERFORMANCE LIQUID-CHROMATOGRAPHY
MOBILITY-MASS SPECTROMETRY
AQUEOUS NORMAL-PHASE
LABELING LC-MS
REVERSED-PHASE
HYDROPHILIC INTERACTION
METABONOMIC ANALYSIS
LARGE-SCALE
TOF-MS
UPLC-MS
Publication Status
Published
Date Publish Online
2019-04-08