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Direct detection of lipid A on intact Gram-negative bacteria by
MALDI-TOF mass spectrometry
File(s)
Author(s)
Larrouy-Maumus, GJ
Abigail Clements
Alain Filloux
Ronan R. McCarthy
Serge Mostowy
Type
Journal Article
Abstract
The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the matrix combined with MALDI-TOF mass spectrometry.
Date Issued
2015-12-03
Date Acceptance
2015-12-02
Citation
Journal of Microbiological Methods, 2015, 120, pp.68-71
URI
DOI
ISSN
1872-8359
Publisher
Elsevier
Start Page
68
End Page
71
Journal / Book Title
Journal of Microbiological Methods
Volume
120
Copyright Statement
© 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license
(http://creativecommons.org/licenses/by/4.0/).
License URL
Publication Status
Published