Fiber bundle shifting endomicroscopy for high resolution imaging
File(s)
Published version
Author(s)
Vyas, K
Hughes, Michael
Gil Rosa, Bruno
Yang, Guang Zhong
Type
Journal Article
Abstract
Flexible endomicroscopes commonly use coherent fiber bundles with high core densities to facilitate high-resolution in vivo imaging during endoscopic and minimally-invasive procedures. However, under-sampling due to the inter-core spacing limits the spatial resolution, making it difficult to resolve smaller cellular features. Here, we report a compact and rapid piezoelectric transducer (PZT) based bundle-shifting endomicroscopy system in which a super-resolution (SR) image is restored from multiple pixelation-limited images by computational means. A miniaturized PZT tube actuates the fiber bundle behind a GRIN micro-lens and a Delaunay triangulation based algorithm reconstructs an enhanced SR image. To enable real-time cellular-level imaging, imaging is performed using a line-scan confocal laser endomicroscope system with a raw frame rate of 120 fps, delivering up to 2 times spatial resolution improvement for a field of view of 350 µm at a net frame rate of 30 fps. The resolution enhancement is confirmed using resolution phantoms and ex vivo fluorescence endomicroscopy imaging of human breast specimens is demonstrated.
Date Issued
2018-09-06
Date Acceptance
2018-07-30
Citation
Biomedical Optics Express, 2018, 9 (10), pp.4649-4664
ISSN
2156-7085
Publisher
Optical Society of America
Start Page
4649
End Page
4664
Journal / Book Title
Biomedical Optics Express
Volume
9
Issue
10
Copyright Statement
© 2018 The Author(s). Published by The Optical Society under the terms of the
Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
Publication Status
Published