Characterization of virus-like particles associated with the human faecal and caecal microbiota
File(s)Phage_paper.zip (4.24 MB)
Accepted version
Author(s)
Type
Journal Article
Abstract
This work represents an investigation into the presence, abundance and diversity of virus-like particles (VLPs) associated with human faecal and caecal samples. Various methodologies for the recovery of VLPs from faeces were tested and optimized, including successful down-stream processing of such samples for the purpose of an in-depth electron microscopic analysis, pulsed-field gel electrophoresis and efficient DNA recovery. The applicability of the developed VLP characterization method beyond the use of faecal samples was then verified using samples obtained from human caecal fluid.
Date Issued
2014-10-13
Citation
Research in Microbiology, 2014
ISSN
1769-7123
Publisher
Elsevier
Journal / Book Title
Research in Microbiology
Copyright Statement
© 2014 Published by Elsevier Masson SAS on behalf of Institut Pasteur. NOTICE: this is the author’s version of a work that was accepted for publication in Research in Microbiology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in RESEARCH IN MICROBIOLOGY, (2014) DOI: 10.1016/j.resmic.2014.10.006
Description
14.11.14 KB. Ok to add accepted version to spiral, subject to 12 months embargo