Improved spatial resolution of metabolites in tissue biopsies using high-resolution magic-angle-spinning slice localization NMR spectroscopy.
File(s)
Author(s)
Type
Journal Article
Abstract
High-resolution magic-angle-spinning 1H NMR spectroscopy (HR-MAS NMR) is a well-established technique for assessing the biochemical composition of intact tissue samples. In this study, we utilized a method based on HR-MAS NMR spectroscopy with slice localization (SLS) to achieve spatial resolution of metabolites. The obtained 7 slice spectra from each of the model samples (i.e., chicken thigh muscle with skin and murine renal biopsy including medulla (M) and cortex (C)) showed distinct metabolite compositions. Furthermore, we analyzed previously acquired 1H HR-MAS NMR spectra of separated cortex and medulla samples using multivariate statistical methods. Concentrations of glycerophosphocholine (GPC) were found to be significantly higher in the renal medulla compared to the cortex. Using GPC as a biomarker, we identified the tissue slices that were predominantly the cortex or medulla. This study demonstrates that HR-MAS SLS combined with multivariate statistics has the potential for identifying tissue heterogeneity and detailed biochemical characterization of complex tissue samples.
Date Issued
2020-09-01
Date Acceptance
2020-07-13
Citation
Analytical Chemistry, 2020, 92 (17), pp.11516-11519
ISSN
0003-2700
Publisher
American Chemical Society
Start Page
11516
End Page
11519
Journal / Book Title
Analytical Chemistry
Volume
92
Issue
17
Copyright Statement
© 2020 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.analchem.0c02377
Sponsor
Medical Research Council (MRC)
Commission of the European Communities
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/32815363
Grant Number
MR/P002536/1
715662
Subjects
Science & Technology
Physical Sciences
Chemistry, Analytical
Chemistry
SPECTRA
KIDNEY
0301 Analytical Chemistry
0399 Other Chemical Sciences
Analytical Chemistry
Publication Status
Published
Coverage Spatial
United States
Date Publish Online
2020-08-20