Cdc42 and Par6-PKC zeta regulate the spatially localized association of Dlg1 and APC to control cell polarization
Author(s)
Etienne-Manneville, S
Manneville, JB
Nicholls, S
Ferenczi, MA
Hall, A
Type
Journal Article
Abstract
Cell polarization is essential in a wide range of biological processes such as morphogenesis, asymmetric division, and directed migration. In this study, we show that two tumor suppressor proteins, adenomatous polyposis coli (APC) and Dlg1-SAP97, are required for the polarization of migrating astrocytes. Activation of the Par6–PKCζ complex by Cdc42 at the leading edge of migrating cells promotes both the localized association of APC with microtubule plus ends and the assembly of Dlg-containing puncta in the plasma membrane. Biochemical analysis and total internal reflection fluorescence microscopy reveal that the subsequent physical interaction between APC and Dlg1 is required for polarization of the microtubule cytoskeleton.
Date Issued
2005-09-12
Date Acceptance
2005-08-02
Citation
The Journal of Cell Biology, 2005, 170 (6), pp.895-901
ISSN
0021-9525
Publisher
Rockefeller University Press
Start Page
895
End Page
901
Journal / Book Title
The Journal of Cell Biology
Volume
170
Issue
6
Copyright Statement
© The Rockefeller University Press 2005
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000231821400007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Life Sciences & Biomedicine
Cell Biology
TUMOR-SUPPRESSOR PROTEIN
ADENOMATOUS POLYPOSIS-COLI
PLASMA-MEMBRANE
MITOTIC SPINDLE
POLARITY
CANCER
DYNEIN
HDLG
PROLIFERATION
MICROTUBULES
Publication Status
Published
Date Publish Online
2005-09-12