A bacteriophage transcription regulator inhibits bacterial transcription initiation by Sigma-factor displacement
Author(s)
Type
Journal Article
Abstract
Bacteriophages (phages) appropriate essential processes of bacterial hosts to benefit their own development. The multisubunit bacterial RNA polymerase (RNAp) enzyme, which catalyses DNA transcription, is targeted by phage-encoded transcription regulators that selectively modulate its activity. Here, we describe the structural and mechanistic basis for the inhibition of bacterial RNAp by the transcription regulator P7 encoded by Xanthomonas oryzae phage Xp10. We reveal that P7 uses a two-step mechanism to simultaneously interact with the catalytic β and β’ subunits of the bacterial RNAp and inhibits transcription initiation by inducing the displacement of the σ70-factor on initial engagement of RNAp with promoter DNA. The new mode of interaction with and inhibition mechanism of bacterial RNAp by P7 underscore the remarkable variety of mechanisms evolved by phages to interfere with host transcription.
Date Issued
2014-04-01
Date Acceptance
2013-12-30
Citation
Nucleic Acids Research, 2014, 42 (7), pp.4294-4305
ISSN
0305-1048
Publisher
Oxford University Press
Start Page
4294
End Page
4305
Journal / Book Title
Nucleic Acids Research
Volume
42
Issue
7
Copyright Statement
(c) The Author(s) 2014. Published by Oxford University Press.
Sponsor
Biotechnology and Biological Sciences Research Council (BBSRC)
Medical Research Council (MRC)
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000334761100024&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Grant Number
BB/K000233/1
G1001664
Subjects
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
COLI RNA-POLYMERASE
BETA-FLAP DOMAIN
CRYSTAL-STRUCTURE
PROTEIN CONTACTS
GENE-EXPRESSION
PROMOTER
SUBUNIT
TERMINATION
RESONANCE
RESIDUES
Publication Status
Published
Date Publish Online
2014-01-28