NMR spectroscopy derived plasma biomarkers of inflammation in human populations: influences of age, sex and adiposity
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Understanding the distribution and variation in inflammatory markers is crucial for advancing our knowledge of inflammatory processes and evaluating their clinical utility in diagnosing and monitoring acute and chronic disease. 1H NMR spectroscopy of blood plasma and serum was applied to measure a composite panel of inflammatory markers based on acute phase glycoprotein signals (GlycA and GlycB) and sub-regions of the lipoprotein derived Supramolecular Phospholipid Composite signals (SPC1, SPC2 and SPC3) to establish normal ranges in two healthy, predominantly white cohorts from Australia (n = 398) and Spain (n = 80; ages 20–70 years). GlycA, GlycB, SPC1 and SPC3 were not significantly impacted by age or sex, but SPC2 (an HDL-related biomarker) was significantly higher in women across all age ranges by an average of 33.7%. A free-living Australian population cohort (n = 3945) was used to explore the relationship of BMI with the panel of inflammatory markers. The glycoprotein signals were directly associated with BMI with GlycB levels being significantly higher for women in all BMI classes. Conversely, SPC2 was found to be inversely associated with BMI and differed significantly between the sexes at each BMI category (normal weight p = 3.46x10-43, overweight p = 3.33x10-79, obese p = 2.15x10-64). SPC1 and SPC3 were markedly less affected by BMI changes. Given the significant association between SPC2 and sex, these data suggest that men and women should be modelled independently for NMR-determined inflammatory biomarkers, or that data should be corrected for sex.
Editor(s)
de Carvalho, Luiz Sérgio Fernandes
Date Issued
2025-01-06
Date Acceptance
2024-09-27
Citation
PLoS One, 2025, 20 (1)
ISSN
1932-6203
Publisher
Public Library of Science (PLoS)
Journal / Book Title
PLoS One
Volume
20
Issue
1
Copyright Statement
© 2025 Lodge et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/39761295
PII: PONE-D-24-24827
Subjects
Humans
Female
Male
Middle Aged
Adult
Biomarkers
Aged
Inflammation
Adiposity
Body Mass Index
Australia
Young Adult
Sex Factors
Age Factors
Magnetic Resonance Spectroscopy
Cohort Studies
Spain
Publication Status
Published
Coverage Spatial
United States
Article Number
e0311975
Date Publish Online
2025-01-06