Next generation histology methods for three-dimensional imaging of fresh and archival human brain tissues
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Modern clearing techniques for the three-dimensional (3D) visualization of neural tissue microstructure have been very effective when used on rodent brain but very few studies have
utilized them on human brain material, mainly due to the inherent difficulties in processing post26
mortem tissue. Here, we develop a tissue clearing solution, OPTIClear, optimised for fresh and archival human brain tissue, including formalin-fixed paraffin-embedded material. In light of
practical challenges with immunostaining in tissue clearing, we adapt the use of cresyl violet for visualization of neurons in cleared tissue, with the potential for 3D quantification in regions of
interest. Furthermore, we use lipophilic tracers for tracing of neuronal processes in post-mortem tissue, enabling the study of the morphology of human dendritic spines in 3D. The development of
these different strategies for human tissue clearing has wide applicability and, we hope, will provide
a baseline for further technique development.
utilized them on human brain material, mainly due to the inherent difficulties in processing post26
mortem tissue. Here, we develop a tissue clearing solution, OPTIClear, optimised for fresh and archival human brain tissue, including formalin-fixed paraffin-embedded material. In light of
practical challenges with immunostaining in tissue clearing, we adapt the use of cresyl violet for visualization of neurons in cleared tissue, with the potential for 3D quantification in regions of
interest. Furthermore, we use lipophilic tracers for tracing of neuronal processes in post-mortem tissue, enabling the study of the morphology of human dendritic spines in 3D. The development of
these different strategies for human tissue clearing has wide applicability and, we hope, will provide
a baseline for further technique development.
Date Issued
2018-03-14
Date Acceptance
2018-02-02
Citation
Nature Communications, 2018, 9
ISSN
2041-1723
Publisher
Nature Publishing Group
Journal / Book Title
Nature Communications
Volume
9
Copyright Statement
© The Author(s) 2018. This article is licensed under a Creative Commons
Attribution 4.0 International License, which permits use, sharing,
adaptation, distribution and reproduction in any medium or format, as long as you give
appropriate credit to the original author(s) and the source, provide a link to the Creative
Commons license, and indicate if changes were made. The images or other third party
material in this article are included in the article
’
s Creative Commons license, unless
indicated otherwise in a credit line to the material. If material is not included in the
article
’
s Creative Commons license and your intended use is not permitted by statutory
regulation or exceeds the permitted use, you will need to obtain permission directly from
the copyright holder. To view a copy of this license, visit
http://creativecommons.org/
licenses/by/4.0/
Attribution 4.0 International License, which permits use, sharing,
adaptation, distribution and reproduction in any medium or format, as long as you give
appropriate credit to the original author(s) and the source, provide a link to the Creative
Commons license, and indicate if changes were made. The images or other third party
material in this article are included in the article
’
s Creative Commons license, unless
indicated otherwise in a credit line to the material. If material is not included in the
article
’
s Creative Commons license and your intended use is not permitted by statutory
regulation or exceeds the permitted use, you will need to obtain permission directly from
the copyright holder. To view a copy of this license, visit
http://creativecommons.org/
licenses/by/4.0/
Sponsor
Parkinson's UK
The Jean Shanks Foundation
Grant Number
K-1403
n/a
Subjects
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
ALZHEIMERS-DISEASE
BASAL FOREBRAIN
NEURONS
RECONSTRUCTION
CONNECTIONS
SYSTEMS
Brain
Catecholamines
Fluorescent Antibody Technique
Humans
Imaging, Three-Dimensional
Immunohistochemistry
Paraffin Embedding
Brain
Humans
Catecholamines
Imaging, Three-Dimensional
Fluorescent Antibody Technique
Immunohistochemistry
Paraffin Embedding
Publication Status
Published
Article Number
1066