Click it or stick it: covalent and non-covalent methods for protein-self assembly
File(s)
Accepted version
Author(s)
Lange, Oskar J
Polizzi, Karen M
Type
Journal Article
Abstract
Protein complexes are ubiquitous in living systems and have a range of biotechnological applications. However, building protein structures from scratch can be a difficult and laborious process. Here, we review recent developments in protein self-assembly, including a range of tools for covalent and non-covalent assembly of protein structures with user-defined architectures. Key achievements in covalent protein assembly include the development of systems with fast reaction rates and nM affinities. Non-covalent assembly methods have lagged because of the complexity of natural interactions governing protein assembly; but recent developments have created modular methods that are more broadly applicable. On the horizon, we foresee an increasing role for computational protein design tools as key in cementing the role of applications, as opposed to methodology, as the main driving force of research in this field.
Date Issued
2021-12
Date Acceptance
2021-08-01
Citation
Current Opinion in Systems Biology, 2021, 28, pp.100374-100374
ISSN
2452-3100
Publisher
Elsevier BV
Start Page
100374
End Page
100374
Journal / Book Title
Current Opinion in Systems Biology
Volume
28
Copyright Statement
© 2021 Elsevier Ltd. All rights reserved
Identifier
https://www.sciencedirect.com/science/article/pii/S2452310021000688
Publication Status
Published
Article Number
100374
Date Publish Online
2021-08-18